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Item Regenerative Competence in Root explants of Medicinally important Orchid Cymbidium aloifolium: An in vitro study(Vegetos: International Journal of Plant Research are the property of SPR, 2018-03-31) Verma, Shivani; Pathak, PromilaThe paper reports the possibility of using root explants for micropropagating Cymbidium aloifolium. Mitra et al. (1976) medium supplemented with 2, 4-D ( 2mgl-1 ) induced callusing at the root tip. However, cent% explants responded via shoot bud formation within 13.25 ± 0.95 days. The neoformations organogenated subsequently. The plantlets grown on M medium supplemented with NAA (3mgl-1 ) and those with 4-5 leaves and a number of roots and further these were subjected to a two-step hardening procedure by subjecting them to in vitro and ex vitro hardening. These survived with 60% frequencyItem Mass Propagation of Cymbidium Bicolor(J. Orchid SOC India, 2013-12-05) Verma, Shivani; Dhiman, KirtiCymbidium bicolor Lindl., a horticulturally important orchid is distributed at an altitude of 400-1000 m from Peninsular India to Srilanka. Its populations are getting rarer due to extensive collections and habitat destruction pressures. The present paper reports the propagation of this species using in vitro asymbiotic seed culture technique with a view to developing an efficient protocol for its mass propagation.The germination potential of its seeds at different stages of development [42, 58 wks after pollination its mit was tested on four ditferent nutrient9761) It was observed and Skoog 1962),PDA (Potato Dextrcse agar, DW + Agar wailled Water+ Agar) and M (Mitra er a/. (197611. It was observed that seeds obtained from capsules (58 wap) invariably failed (Distirw any germination response whereas seeds procured from capsules at another stage (42 wapl positively responded to o iation. Since, germination frequency w t in M medium; the germination potantial of these seeds was geneequently assessed on M medium with growth additives (IAA, IBA, NAA, BAP, at1 mgl each; P, CH, AC at 2 gt each). Healthy seedlings complete with 2-3 leaves and 1-2 roots were transferred to green house with 70% survival. Based on the present observations, different nutritional combinations are suggested for germination (M), protocorm growth and mutiplication (M+AC), early differentiation stages (M+P+AC; M+AC) and seedling growth and maintenance (M+P)Item Effective use of synthetic seed technology in the regeneration of Cymbidium aloifolium using protocorm-like bodies(CURRENT SCIENCE VOL 120, 2021-02-10) Verma, Shivani; Pathak, PromilaSynthetic seed technology offers tremendous potential in micropropagation. It deals with in vitro conservation and storage of rare and endangered plant species along with their easy handling and transportation. This technology is becoming prevelant due to its wide applications in germplasm conservation and for exchanges between countries in floricultural trade. The present study examines the regeneration and conversion capabilities of Cymbidium aloifolium using protocorm-like bodies when stored at different temperatures. The propagules showed high proliferative potential by multiplication and complete plantlets were obtained in 58 days on basal M medium supplemented with 1 mg l–1 of indole-3-acetic acid.