Regenerative Competence in Root explants of Medicinally important Orchid Cymbidium aloifolium: An in vitro study
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Date
2018-03-31
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Vegetos: International Journal of Plant Research are the property of SPR
Abstract
The paper reports the possibility of using root explants for
micropropagating Cymbidium aloifolium. Mitra et al. (1976) medium
supplemented with 2, 4-D ( 2mgl-1
) induced callusing at the root tip.
However, cent% explants responded via shoot bud formation within
13.25 ± 0.95 days. The neoformations organogenated subsequently.
The plantlets grown on M medium supplemented with NAA (3mgl-1
)
and those with 4-5 leaves and a number of roots and further these were
subjected to a two-step hardening procedure by subjecting them to in
vitro and ex vitro hardening. These survived with 60% frequency