Mass Propagation of Cymbidium Bicolor
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Date
2013-12-05
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J. Orchid SOC India
Abstract
Cymbidium bicolor Lindl., a horticulturally important orchid is distributed at an altitude of 400-1000 m from Peninsular India to
Srilanka. Its populations are getting rarer due to extensive collections and habitat destruction pressures. The present paper reports
the propagation of this species using in vitro asymbiotic seed culture technique with a view to developing an efficient protocol for
its mass propagation.The germination potential of its seeds at different stages of development [42, 58 wks after pollination
its mit was tested on four ditferent nutrient9761) It was observed and Skoog 1962),PDA (Potato Dextrcse agar, DW + Agar wailled Water+ Agar) and M (Mitra er a/. (197611. It was observed that seeds obtained from capsules (58 wap) invariably failed
(Distirw any germination response whereas seeds procured from capsules at another stage (42 wapl positively responded to
o iation. Since, germination frequency w t in M medium; the germination potantial of these seeds was geneequently assessed on M medium with growth additives (IAA, IBA, NAA, BAP, at1 mgl each; P, CH, AC at 2 gt each). Healthy seedlings complete with 2-3 leaves and 1-2 roots were transferred to green house with 70% survival. Based on the present observations, different nutritional combinations are suggested for germination (M), protocorm growth and mutiplication (M+AC), early differentiation stages (M+P+AC; M+AC) and seedling growth and maintenance (M+P)