Browsing by Author "Pathak, Promila"

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    Effective use of synthetic seed technology in the regeneration of Cymbidium aloifolium using protocorm-like bodies
    (CURRENT SCIENCE VOL 120, 2021-02-10) Verma, Shivani; Pathak, Promila
    Synthetic seed technology offers tremendous potential in micropropagation. It deals with in vitro conservation and storage of rare and endangered plant species along with their easy handling and transportation. This technology is becoming prevelant due to its wide applications in germplasm conservation and for exchanges between countries in floricultural trade. The present study examines the regeneration and conversion capabilities of Cymbidium aloifolium using protocorm-like bodies when stored at different temperatures. The propagules showed high proliferative potential by multiplication and complete plantlets were obtained in 58 days on basal M medium supplemented with 1 mg l–1 of indole-3-acetic acid.
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    Regenerative Competence in Root explants of Medicinally important Orchid Cymbidium aloifolium: An in vitro study
    (Vegetos: International Journal of Plant Research are the property of SPR, 2018-03-31) Verma, Shivani; Pathak, Promila
    The paper reports the possibility of using root explants for micropropagating Cymbidium aloifolium. Mitra et al. (1976) medium supplemented with 2, 4-D ( 2mgl-1 ) induced callusing at the root tip. However, cent% explants responded via shoot bud formation within 13.25 ± 0.95 days. The neoformations organogenated subsequently. The plantlets grown on M medium supplemented with NAA (3mgl-1 ) and those with 4-5 leaves and a number of roots and further these were subjected to a two-step hardening procedure by subjecting them to in vitro and ex vitro hardening. These survived with 60% frequency