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Browsing Research Articles by Author "Sandeep Sharma"

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    Conservation of Picrorrhiza kurroa with arbuscular mycorrhizal fungi: An endangered and highly economic medicinal herb of Himalaya
    (Indianjournals.com Medicinal Plants Vol. 12 (1), March 2020, 33-40, 2020) Sapana Sharma1; Sandeep Sharma; Vivek Sharma; M.J. Singh
    Arbuscular mycorrhizal (AM) fungi are prevalent from arctic to tropics in most agricultural and natural ecosystems, play an important role in the conservation of some valuable medicinal plants. Picrorrhiza kurroa Royle ex Benth is distinguished endangered medicinal herb, naturally distributed in alpine and sub-alpine region of Himalayas. In present investigation, spores density of AM fungi along root colonization were studied from the rhizospheric soils of P. kurroa collected from different locations of Himachal Pradesh. Highest spore number (60) was found in the soil samples collected from forest nursery Rajkunda while AM root space colonization was highest in the samples of forest nursery Devidhar. The biodiversity of AM fungi was studied; the number of AM species was also highest in these two forest nursery samples. Funneliformis mosseae and Acaulospora laevis were dominant species associated with P. kurroa. After mass multiplication of F. mosseae and A. laevis, bio inoculation experiment was performed on Picrorrhiza kurroa seedlings for the biomass production and nutrient uptake analysis. Seedlings were inoculated with single and in combined mycorrhizal treatments with different combinations of farmyard manure and vermicompost. All inoculated seedlings showed significant biomass production than control seedlings. Root length (8.7cm), fresh and dry biomass was observed to be maximum in the plants inoculated with F. mosseae and vermicompost (Vc) @ 10t ha-1 while percent AM root colonization was highest (60.33) in the roots of plants inoculated with F. mosseae plus A. laevis with vermicompost @ 10t ha-1. The phosphorus content was more in seedlings inoculated with consortium of two native AM fungi followed by F. mosseae and A. laevis, separately. In overall, consortium of native AM fungi i.e. F. mosseae and A. laevis was found to be best for the conservation of the P. kurroa in the presence of vermicompost and FYM, respectively.
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    PERIODIC CHANGES IN ARBUSCULAR MYCORRHIZAL FUNGI IN THE RHIZOSPHERIC SOIL OF FRUIT PLANTS
    (Agric Res J 55 (4) : 685-690, 2018) Sapana Sharma; Sandeep Sharma; Anirudh Thakur; M J Singh
    Arbuscular mycorrhizal (AM) fungi are ubiquitous soil microbes and play a very important role in terrestrial ecosystems. The present study involved analyzing seasonal variation of root colonization and diversity of arbuscular mycorrhizal (AM) fungi in the rhizosphere soil of seven different fruit plants of north-western India. The AM fungal colonization fluctuated seasonally in the rhizosphere of these fruit plants. The total AM fungal colonization (35.0), spore population (31.7) and number of AMF species (5.0) associated was highest in amla (Emblica officinalis) plants in monsoon and post monsoon season. There was significant difference of the colonization by arbuscules and vesicles between the three seasons (pre-monsoon, monsoon and post monsoon). Glomus was found to be the dominant genus in all the plants. Other AMF species of genera Acaulospora, Gigaspora, Sclerocystis and Scutellospora were also present. Different AM colonization structures like mycelium, arbuscules and vesicles were also observed. Wide variations were observed in root colonization, spore number and AM fungal diversity among different plants and seasons.